Fig. 1. Expression of Pax2 in E11.5 metanephric mesenchyme. Whole-mount antibody staining with antibodies against Pax2 (red) and pan-cytokeratin (green) in wild-type (+/+) and Ret-/- mutants as indicated. Pax2 protein is localized to the nephric duct (ND), ureteric bud (UB) and metanephric mesenchyme (MM) in wild-type E11.5 kidneys. Anti-pan-cytokeratin stains the epithelium of the nephric duct and ureteric bud only. Note the expression of Pax2 in the mesenchyme of Ret mutants despite the absence of any ureteric bud. Scale bars: 80 µm.
