Fig. 5. Genetic rescue of the lab mutant brain phenotype by transgenic expression of the Ubx protein (A-C), and failure of genetic rescue of the lab mutant brain phenotype by transgenic expression of the Abd-B protein (D-F) in a lab null mutant background. Laser confocal microscopy of stage 15 embryos, reconstructions of optical sections. (A,D) Frontal views, (B,C,E,F) lateral views. (B,C) From the same preparation; (E,F) from the same preparation. (A,B,D,E) Anti-HRP immunolabeling. Arrows indicate location of circumesophageal connectives, arrowhead indicates location of tritocerebral commissure. (C) Double immunolabeling with anti-HRP (red) and anti-Ubx (green). Arrow indicates targeted misexpression domain of Ubx in the lab mutant embryonic brain (equivalent to the endogeneous expression domain of Lab in the wild-type embryonic brain). Asterisk labels part of the endogenous Ubx expression domain in the ventral nerve cord. (D) Double immunolabeling with anti-HRP (red) and anti-Abd-B (green). Arrow indicates targeted misexpression domain of Abd-B in the lab mutant embryonic brain (equivalent to the endogeneous expression domain of Lab in the wild-type embryonic brain). The endogenous Abd-B expression domain in the ventral nerve cord is located in posterior neuromeres that are not shown.
