Fig. 2. The Scl 3' enhancer construct targets lacZ expression to c-Kit+CD34+ cells from the AGM region. Cell suspensions from the AGM region of +6E5/lacZ/3'En transgenic E11 embryos were stained with biotinyl-conjugated anti-CD34 and PE-conjugated anti-c-Kit antibodies followed by streptavidin-FITC. Cells were sorted according to indicated windows. After sorting, cytospin preparations were stained for ß-galactosidase. In the representative experiment shown, approximately 70% of the c-Kit+CD34+ cells were lacZ+, compared with fewer than 1% of the c-Kit+CD34– population. Dead cells were excluded by PI staining.
