Fig. 1. Expression of pnr during embryonic development. (A,B) Lateral view (anterior left, dorsal up) of early embryos (stages 5-6) showing the distribution of pnr transcripts, and Eve (A) and Ftz (B) proteins. pnr expression covers a broad dorsal domain. The anterior limit is close to the second eve stripe, although there is some low level expression anterior to the eve stripe, which is not visible in the picture. The posterior limit coincides well with the anterior border of the seventh Ftz stripe. (C) Dorsal view of a late embryo (stage 13) doubly labelled for pnr mRNA and Cad protein. pnr transcripts lack in the amnioserosa region (am) and in the A8 segment. There is pnr expression in A9, but not in A10, where cad is expressed. (D,E) Confocal images of lateral view of en-lacZ embryos doubly stained with anti-Pnr antibody and anti ß-gal. The spotty appearance of Pnr label indicates the protein is in the cell nucleus. There is Pnr protein in the A8 segment in stage 10 (D), but not in stage 11 (E). (F) Lateral view of a stage 12 embryo showing pnr and dpp expression. The dorsal dpp stripe is within the Pnr domain and shares the same dorsal limit at the junction with the amnioserosa.
