Fig. 2. Structure of the lhp1-1 locus. (A) Localisation of the 1.2 kb deletion on the genomic MVA3 P1 clone which accompanied the T-DNA region insertion in lhp1-1. Database searches indicated two putative genes in this region, LHP1 and TMP. The plasmids, pCaS, pCaES and pCaSSP, were used for complementation experiments. (B) Detail of the T-DNA insertion in lhp1-1. Insertion occurs at position 11954, 22 bp upstream of the 5' end of the isolated cDNA (11932). At the T-DNA right-border::genomic DNA junction, the integration was accompanied by deletions of the 24 bp RB repeat and the 21 bp adjacent to it. A small 47 bp insertion with no identified homology was also detected. The T-DNA LB was better conserved than the RB as commonly observed during T-DNA integration. Numbers refer to the sequence of the MVA3 P1 clone (accession number AB006706).
