Fig. 4. Sequence comparisons of the chromo (A) and chromo shadow domains (B). Arabidopsis AtLHP1 (present work), B. rapa BrLHP1 (the EST sequence is likely truncated at the 5' end), Daucus carota DcCB1 (D83719), Drosophila melanogaster HP1 (DmHP1; AAA28620) and Polycomb (DmPC; A38565), Homo sapiens HP1
(HuHP1, P45973) and Mus musculus HP1 (MmMOD1; P23197). The positions of the regions used for alignments and identity scores between AtLHP1 and different CD/CSD are indicated. Based on NMR studies, ß-sheet and -helix secondary structures are indicated above the alignments (Ball et al., 1997; Brasher et al., 2000). The residues that form the hydrophobic core are highlighted in yellow. (A) Residues glycine 34 and 44 and proline 54, playing a crucial role in the tertiary CD structure, are indicated (green star). A black star indicates the position of the Y24F mutation in DmHP1. (B) Residues involved in dimerisation (with the main contacts being at MmMOD1 I161, Y164 and L168) are highlighted in purple.
