Fig. 5. LHP1 homodimerisation in the yeast two-hybrid system. (A) Schematic representations of LHP1 and truncated LHP1 proteins. LHP1N: N-terminal region (aa 1-194). LHP1C: long C-terminal region (aa 162-445). LHP1CSD: short C-terminal region (aa 378-445). (B,C) Growth of different yeast strains containing combinations of fusion proteins. Selective media lacking particular amino acids (L: leu, W: trp, H: his) or rich YPD medium lacking adenine are indicated under each plate. On medium lacking adenine, interacting proteins result in white colonies, non-interacting proteins results in red colonies. X-Gal plates show staining for ß-galactosidase activity. 1, positive control. The plasmids pTD1-1 and pVA3-1 encoding proteins p53 and SV40 are known to interact in vivo. 2, pBD-LHP1 and pAD-LHP1. 3, pBD-LHP1 and pTD1-1. 4, pAD-LHP1 and pVA3-1. 5, pBD-LHP1N and pAD-LHP1N. 6, pBD-LHP1C and pAD-LHP1C. 7, pBD-LHP1CSD and pAD-LHP1CSD.
