Fig. 7. LHP1 has a specific subnuclear localisation in tobacco mesophyll protoplasts, in transient assays. Chloroplasts appear red and GFP fluorescence is green; when the two fluorescences overlap, the yellow colour appears. (A,B) Protoplasts expressing GFP alone (pAVA121 plasmid). (A) Projection. (B) Section. (C,D) Protoplast expressing GFP-VirD2NLS. (C) Projection. (D) Section. The GFP fluorescence is uniformly distributed throughout the nucleus. (E-H) Protoplasts electroporated with the LHP1-GFP construct. A diffuse nucleoplasmic distribution and discrete particles are observed. (E) Projection of several protoplasts. One protoplast expresses the LHP1-GFP fusion in the nucleus, the others are not transformed. (F) Close-up view of the nucleus (projection). (G-H) Sections. Scale bar, (A-E) 10 µm; (F-H) 2 µm.
