Fig. 2. Protein levels of paranodin, Na⁺ and K⁺ channel subunits in the brain of wild-type and MBP-TK mice treated from day 1 to 20. Forebrain tissue from chronically treated (1-20d) wild-type and MBP-TK mice were homogenized as described in the Methods, and equal amounts of proteins were analyzed by immunoblotting (10 µg for paranodin or 30 µg for K⁺ and Na⁺ channels). No statistical differences in the levels of paranodin (180 kDa), Na⁺ channel subunit (260 kDa) and K⁺ channels (86 kDa) were observed after quantification of the western blots (less than 10% variation between samples). The results are representative of three different experiments.

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