Fig. 3. Immunofluorescence studies of MBP and PND/Caspr in the corpus callosum and the cortex of wild-type and treated MBP-TK mice. Myelinated fibers in the cortex and the corpus callosum are strongly labeled by the MBP antibody in wild-type animals (A). Absence of cortical MBP-positive fibers and a significant reduction of MBP labeling is observed in the corpus callosum of MBP-TK mice (1-20d) (B). Arrowhead in B indicates residual myelinated axons in the first cortical layer of MBP-TK mice brain. MBP-positive myelinated fibers are less abundant in MBP-TK mice corpus callosum after 1-6d treatment (C) than after 6-20d treatment (D). (E,F) Double immunostaining using anti-MBP (red) and anti-paranodin (green) antibodies. The presence of myelin sheaths correlates with the focal localization of paranodin at the paranodal zone in a wild-type animal (E). Paranodin staining is concentrated at the paranode only in myelinated fibers (arrow, F). Scale bars: in D, 100 µm for A-D; in F 10 µm for E,F.
