Fig. 5. Localization of Na+ and K+ channels in jimpy mice. Sections from corpus callosum of wild-type (A,C) or jimpy (B,D) littermates were examined by double immunostaining with antibodies against the Na+ channels (green) and the K+ channels Kv1.1 (red). Postnatal day 15 (A,B). In wild-type mice, Kv1.1 immunoreactivity was clearly separated from Na+ channels clusters by an empty space corresponding to paranodes (A). In jimpy mice, although the shape of Na+ and K+ channel clusters was roughly comparable with that in wild-type littermates, the localization of Kv1.1 immunoreactivity was abnormal as it was not separated from the nodal Na+ channels (arrowhead, B). Postnatal day 21 (C,D). The alteration of K+ channels distribution was much more severe than at P15. Immunoreactivity appeared more diffuse, and Kv1.1 aggregates were irregular and fragmented. The shape of Na+ channels clusters was also altered with fuzzy and irregular limits. Scale bar: 10 µm.
