Fig. 5. Vacuolisation of phosphatase-deficient osteoclasts shown in TEM of post-osmicated, uranyl and lead stained ultra-thin sections. (A) Intracellular vacuole (arrow) in an osteoclast of a LAP-deficient mouse. Note the content of moderate electron density of the vacuole. (B,C) Vacuoles (arrows) in the cytoplasm of an osteoclast of a Acp5-deficient mouse (B) and in an osteoclast from a doubly deficient mouse (C): the vacuoles have a higher electron density and contain linear features that may represent mineral crystallites. (D) High magnification of typical mineral cystallites containing vacuoles of a doubly deficient osteoclast. Scale bars: 0.5 µm in A-C; 130 nm in D.
