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Fig. 7. Rib protein expression and localization. Wild-type (A,C,E) and ribex12 (F) embryos stained with anti-Rib (P3) antiserum Ab21802K. No Rib expression is detected in mutant (F). (B,D) rib RNA distribution at similar stages for comparison. st, stomodeum; ms, mesoderm; pr, proctodeum; ep, epidermis; as, amnioserosa. (G-I) Detail of ventral epidermis in stage 14 embryo double stained with Rib antiserum (fluorescein) and DAPI. (G) DAPI channel. (H) Rib (fluorescein) channel. (I) Merged image. Rib staining is coincident with DAPI except for the centromeric region (arrowhead) which stains intensely only with DAPI. Scale bar: 30 µm in A-F; ~2 µm in G-I.





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