Fig. 6. Rates of chlorination and methylation of [3H]THPH by living cells. (A) Rate of chlorination of THPH by disaggregated slug cells or the prestalk and prespore cells purified from them. (B) Rate of methylation of [3H]THPH by disaggregated slug cells or the prestalk and prespore cells purified from them. (C) Effect of 100 nM DIF-1 or 100 nM DIF-3 on chlorination and methylation by disaggregated slug cells and the prestalk and prespore cells separated from them. Cells were incubated at 107/ml in 1 ml NS/MES containing 1.9 nM [3H]THPH and 40 µM ancymidol (to inhibit breakdown of DIF-3). After 10 minutes at 22°C, labelled compounds were extracted, concentrated and resolved by HPLC, together with unlabelled standards (see Materials and Methods for the method of calculating the chlorination and methylation rates). The prespore fraction was 96% and the prestalk fraction 88% pure.
