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Fig. 6. Analysis of cell identity and cell mingling in embryonic chimaeras. All the pictures show flat-mounted hindbrains. (A) Combined X-gal staining and NeoR ISH performed on a 15 s wild-type/Krox20lacZ/lacZ chimaeric embryo. The white arrowheads point to groups of blue cells in even-numbered rhombomeres. (B) Combined X-gal staining and NeoR ISH performed on a 18 s wild-type/Krox20lacZ/+ chimaeric embryo. (C) X-gal staining of a 14 s chimaeric embryo between wild-type and Epha4/lacZ transgenic lines. (D) Epha4 ISH performed on a 15 s wild-type embryo. (E,F) Combined X-gal staining and Epha4 ISH performed on 15 s (E) and 12 s (F) wild-type/Krox20lacZ/lacZ chimaeric embryos. (G) Combined X-gal staining and NeoR ISH performed on a 9.5 dpc wild-type/Krox20lacZ/Cre R26R chimaeric embryo. (H) Combined X-gal staining and double Krox20 (orange) and Hoxb1 (purple-brown) ISH performed on a 14 s wild-type/Krox20lacZ/lacZ chimaeric embryo. (I) Combined X-gal staining and Hoxb1 ISH performed on a 12 s wild-type/Krox20lacZ/lacZ chimaeric embryo.





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