Fig. 3. Cells in caudal (A,D), rostral (B,E) and dorsal (C,F) E9.5-derived telencephalic explants stained with O4 mAb at 13 days in vitro. (A,B) Note that the morphology of O4+ cells in caudal and rostral explants is identical. Inset in B is a rostral culture stained at 15 DIV with O1 mAb, showing that cells generated in rostral explants are also O1+ and therefore differentiated oligodendrocytes. (C) The vast majority of explants derived from the dorsal telencephalon did not generate O4+ cells after 13 days in vitro. This inability was, however, only transient, and O4+ cells were observed at 19 days in vitro (inset in C). (A-C) O4 labeling; (D-F) corresponding interferential contrast images. Scale bar: 35 µm; 50 µm in the inset.
