Fig. 5. Development of oligodendrocytes in rostral telencephalon depends on Sonic hedgehog signaling. Explants derived from E10.5 rostral telencephalon were analyzed by double immunostaining with O4 (A,B) and TuJ1 (C,D) mAbs at 12 days in vitro. In control experiments, a large number of O4+ (A) and TuJ1+ (C) cells developed. When similar explants were grown in the presence of a function blocking anti-Shh antibody for 12 days, no O4+ cells were detected (B); however, TuJ1+ cells still developed (D), although less efficiently than in control (C). Scale bar: 130 µm.
