Fig. 2. Early embryonic expression pattern of Hmx2 revealed by staining Hmx2lacZ+/– whole mounts for ß-galactosidase. Embryonic stages are indicated at the lower-right corner of each panel. ß-galactosidase expression patterns are identical to that obtained by RNA in situ hybridization. (A) No ß-galactosidase activity can be detected at E8.5. Hmx2 is first turned on at E9.0. (B) At E9.5 and (C) E10.5, Hmx2 expression becomes more prominent in the anterior portion of otic vesicle and otocyst, respectively and the cleft between the first and second branchial arches. (D) At E12.0, expression of Hmx2 can also be seen in the developing neural tube and hypothalamus. Arrows indicate the positions of the otic anlagen. The arrowhead in B indicates the Hmx2 expression in the cleft between the first and second branchial arches. Arrowheads in D indicate the Hmx2 expression in the central nervous system. Letters AB, CD and EF indicate the level and orientation of corresponding sections shown in Fig. 6.
