Fig. 2. unc-83 mutations do not disrupt centrosome attachment to the nucleus. Dorsal views through the intestinal primordium (A,B) or through hyp7 precursors (C) in pre-elongation embryos grown at 15°C. Anterior is leftwards. (A) Wild-type embryo. (B,C) unc-83(e1408) embryos. Embryos were stained with the IFA antibody to identify centrosomes (pseudocolored red) and DAPI to identify chromatin (pseudocolored blue). hyp7 precursors were identified using the jam-1::GFP marker to show hypodermal cell boundaries, pseudocolored green. M marks the midline where the intestinal lumen will form. The arrows mark examples of nuclei with centrosomes properly associated. Note that for the wild-type embryo, nuclei have migrated toward the midline, but nuclei are separated away from the midline in the unc-83(e1408) embryo. Because of the limited focal plane of these images, only a subset of nuclei or centrosomes are shown. Scale bars: in B, 5 µm for A,B; in C, 5 µm for C.
