Fig. 5. UNC-83 is localized to the nuclear envelope. In all panels, anterior is leftwards, and dorsal is upwards. UNC-83 antibody localization is pseudocolored red, and DAPI staining is in blue. In some panels, JAM-1::GFP is used to show adherens junctions in green. (A-C) Pre-elongation embryos, examples of hyp7 nuclei are marked with arrowheads. (A) unc-83(e1408), a putative null. (B) Wild-type. (C) unc-83(ku18), an allele in which hyp7 nuclear migration is not disrupted. (D-F) Lateral views of sections through a single wild-type embryo at the onset of elongation (bean stage). (D) A section near the top with P-cell nuclei marked by arrowheads. (E) A section 1 µm lower showing hyp7 nuclei marked with arrowheads. (F) A section 4 µm lower showing UNC-83 staining in intestinal cells marked with arrowheads. (G-I) Wild-type worms. (G) A lateral view of a hermaphrodite late in the first larval stage showing UNC-83 in marked P cells. The arrow marks an intestinal cell in the same plane. (H) The tail of an adult worm. UNC-83 stained many hypodermal cells, examples are marked with arrowheads, a large intestinal cell (arrow) and other unidentified cells. (I) The head of an adult worm showing UNC-83 staining in hypodermal (examples marked with arrowheads) and other unidentified cells. Scale bars: in F, 10 µm for A-F; in G, 9 µm for G; in I, 25 µm for H,I.
