Fig. 4. Expression of retinal transcription factors in wild-type (top panels) and in OVE1070 transgenic (bottom panels) embryos. In situ hybridization was performed to assay for expression of Rax (Rx) at E10.5 (A,B), Chx10 at E11.5 (C,D), Pax6 (E, F) and Atoh7 (Math5) (G,H) at E13.5, and Mitf at E11.5 (I,J). Expression of neuronal genes (Rx, Chx10, Pax6 and Math5) was activated in the RPE-derived neural retina (rNR), while Mitf expression was downregulated. In wild-type eyes (E), Pax6 was expressed in the lens epithelium and neural retina, and in the anterior (white arrowheads) but not in the posterior region (yellow arrowheads) of the RPE. D-V, dorsal-ventral orientation; L, lens; LP, lens pit; L, lens vesicle. Scale bars: 50 µm in A-D,I,J; 100 µm in E-H.
