Fig. 5. Induction of neural retina from the presumptive RPE by TRP2-rasT24. Hematoxylin/Eosin staining of an E16.5 transgenic embryo showed that nearly the entire presumptive RPE was converted to a second neural retina (rNR) (A). Antibody labeling (green fluorescence) using anti-ß-tubulin III indicated that ganglion cells had differentiated in both the endogenous neural retina (NR) and rNR (B, boxed region in A). BrdU incorporation showed that numerous cells in the rNR were actively proliferating, analogous to the cells in the original neural retina (NR) (C). Postmitotic ganglion cells (g) were readily visible in the NR, but not as prevalent in the rNR (red arrowhead). Blood cells (arrows in B,C) showed autofluorescence. L, lens. Scale bars: 50 µm in B,C; 100 µm in A.
