Fig. 5. Deduced amino acid sequence of Rols7 and Rols6. From the analysis of the cDNA sequences, the possible open reading frames were deduced. According to conceptual translation, rols6 encodes a protein of 1670 amino acids, 79 of which at the very N terminus are unique to Rols6. rols7 encodes a protein of 1900 amino acids, 309 of which are unique to Rols7. The amino acid sequence of Rols7 is shown in total. The beginning of the common part of Rols7 and Rols6 is indicated by a black triangle, which corresponds to the first common intron-exon boundary of the mRNAs. Structural motifs were determined using the SMART-tool at the EMBL-server at Heidelberg (http:/smart.embl-heidelberg.de) (Schultz et al., 1998; Schultz et al., 2000). This analysis predicts four structural motifs within the common part of both proteins which are underlined and nominated in the sequence: a RING-finger motif in the N-terminal part, a predicted nucleotide binding motif, nine ankyrin motifs and a TPR-repeat (broken underline) close to the C terminus.
