Fig. 3. UO126 blocks FGF-induced phosphorylation of p42/p44 MAPK. (A) Representative immunoblots of phospho-ERK (top) and total ERK (bottom) from lens epithelial explants exposed to no FGF2 (lanes 1 and 3) or 100 ng/ml FGF2 (lanes 2 and 4) for 15 minutes at 37°C, in the absence (lanes 1 and 2) or presence (lanes 3 and 4) of UO126. (B) Lens explant exposed to 100 ng/ml of FGF2 in the presence of UO126, immunolabelled for phospho-ERK (right), counterstained with Hoechst dye (left). Control explants were treated with an equivalent volume of DMSO without UO126. Scale bar: 40 µm.
