Fig. 8. UO126 blocks FGF-induced upregulation of filensin expression, which accompanies fibre differentiation. Representative micrographs of cells in lens explants cultured for 5 days in the absence of FGF2 (A-C) or with 100 ng/ml FGF2 (D-F) in the presence of DMSO (A,B,D,E) or DMSO with UO126 (C,F), immunolabelled for filensin (B,C,E,F) or counterstained with Hoechst dye (A,D). FGF2 induced a marked increase in filensin expression (E). In the presence of UO126, no reactivity was observed in response to FGF2 (F), with levels comparable with control explants (C). Scale bar: 50 µm.
