Fig. 9. UO126 does not block FGF-induced ß-crystallin expression, which accompanies fibre differentiation. Representative micrographs of cells in lens explants cultured for 5 days in the absence of FGF2 (A-C) or with 100 ng/ml FGF2 (D-F) in the presence of DMSO (A,B,D,E) or DMSO with UO126 (C,F), immunolabelled for ß-crystallin (B,C,E,F) or counterstained with Hoechst dye (A,D). FGF2 induces a marked increase in ß-crystallin expression (E) with little to no reactivity detected in control explants (B). In the presence of UO126, although the cells retain an epithelial morphology in response to FGF2, they express ß-crystallin (F). Scale bar: 40 µm.
