Fig. 8. Replication initiation proteins are mis-localized in E2f2 mutant follicle cell nuclei. Ovaries were dissected, fixed and treated with anti-ORC2 (left panels), anti-ORC5 (middle panels) or anti-CDC45L (right panels) antibodies. An image of a single nucleus from a stage 10B egg chamber is shown in each panel. (A) yw67 wild type. These three proteins are detected in a focus coincident with the third chromosome chorion cluster. (B) Df(2L)E2f2329/Df(2L)E2f2329; P[E2f2–; Mpp6+]/+. In the mutant, these replication proteins are not localized. (C) Df(2L)E2f2329/Df(2L)E2f2329; P[E2f2+; Mpp6+]/+. Mis-localization in the mutant is rescued by a wild-type E2f2 transgene.
