Fig. 1. Tissue-specific mosaic screen for mutations in the hh pathway. (A) A two-step screen was designed to identify genes that positively regulate the hh pathway. In the primary screen, mosaic flies were generated using the eyFLP system and mutations that caused hh-like small-eye phenotypes were isolated in the F1 generation. (B) In the secondary screen, mosaic flies bearing large mutant clones in the wing were generated using hsFLP in conjunction with the Minute technique, and were screened for hh-like wing phenotypes. For details, see Results and Materials and Methods. The asterisk indicates a newly induced recessive mutation.
