Fig. 3. cmn affects Hh signaling. In this and the following figures, all the wing discs are oriented with anterior towards left and ventral up. (A) A wild-type wing and (B) a mosaic wing bearing large cmn mutant clones. The central structures of the wing including vein 3 and vein 4 are completely lost. Vein 2 and vein 5 are incomplete. The most anterior (anterior to vein 2) and most posterior (posterior to vein 5) structures are intact. (C,D) Third instar, wild-type (C) and cmn homozygous (D) wing discs were stained with anti-Dpp antibody. Dpp expression near the AP compartment boundary is diminished in the cmn mutant disc (indicated by the arrowheads). (E,F) Wild-type (E) and cmn homozygous (F) wing discs were stained with anti-Ptc antibody. Ptc upregulation is lost in the cmn mutant disc (arrowheads) (G,H). Wild-type (G) and cmn homozygous (H) wing discs were stained with anti-ß-gal antibody to visualize hh-lacZ expression. hh-lacZ expression appears normal in the cmn mutant disc.
