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Fig. 8. cmn is required for the activity of cholesterol-free Hh. Late third instar wing discs of the following genotypes were stained with anti-Ptc to monitor the up-regulation of ptc in response to Hh. (A) Wild type, (B) act>CD2>Gal4/UAS-HhN, (C) disp l(3)S037707, (D) act>CD2>Gal4/UAS-HhN; disp l(3)S037707, (E) cmnM82, (F) act>CD2>Gal4/UAS-HhN; cmnM82. Misexpressing HhN in both wild-type and disp mutant discs induced ectopic ptc upregulation (B,D). In contrast, misexpressing HhN in cmn mutant disc failed to upregulate ptc expression (F).





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