Fig. 4. An anterior domain of lower nuclear densities is formed before the start of cellularization. Embryos are stained against Myosin (red) and DNA (green). First column: cross-section showing absence of cellularization front. Second column: focal plane though nuclei. Numbers across the top represent nuclei counts in a square of constant size (70x70 pixel box) positioned at corresponding points along the AP axis of the embryo. (A) A pre-cellularization, cycle 14 wild-type embryo shows a lower density of nuclei in the anterior. (B) A late-stage cellularizing embryo shows a similar anterior nuclear density as in A, but also has an additional slight clustering of nuclei in the region of the pre-CF domain (arrow).
