Fig. 6. Explant culture experiment: molecular segmentation markers are not expressed in the absence of medial PSM. Dorsal view of medial (right) and lateral (left) PSM explants, generated as described in Fig. 5 and subsequently hybridised with probes for Delta-1 (A), Notch-1 (B), paraxis (C), hairy1 (D), hairy2 (E), lunatic fringe (F), BMP-4 (G), Noggin (H) and Tbx6L (I) antisense RNA probes. The expression of molecular segmentation markers (Delta-1, Notch-1 and paraxis) as well as cycling genes (hairy1, hairy2 and lunatic fringe) is affected in the isolated lateral PSM cells. Nevertheless, the usual expression patterns of Tbx6L, BMP-4 and Noggin are maintained. Scale bar: 300 µm.
