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Fig. 7. Expression of two copies of Gli1 from the Gli2 allele does not result in spinal cord patterning defects. Immunoflurorescent staining of Shh, HNF3ß, Nkx2.2, Isl1/2, Pax6 and Pax7 in E10.5 Gli21ki/+ embryos (A-F), or Gli21ki/1ki embryos (G-L). The size of each mutant used for the analysis was slightly different. White arrowhead indicates the ventral limit of the Pax6 or Pax7 expression domains. Margins of the spinal cord and spinal canal are outlined by broken lines. Scale bar: 100 µm.





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