Fig. 3. GPI isozyme analysis. (A) Representative pattern of GPI assay in the control and plc-
1–/– chimeric mice. Lysates from several tissues isolated from the chimeric mice were separated by cellulose acetate electrophoresis and stained for GPI activity as described in Materials and Methods. RB, red blood cells; BM, bone marrow, SM, skeletal muscle; Te, testis; Ep, epididymis; Bl, bladder; Ap, appendix; Lin, large intestine; Sin, small intestine; Sp, spleen; Pa, pancreas; St, stomach; Ki, kidney; Ad, adrenal; Li, liver; Di, diaphragm; Lu, lung; He, heart; Th, thymus; Es, esophagus; SG, submaxillay gland; TG, thyroid gland; To, tongue; Ey, Eye; FB, forebrain; Ce, cerebellum; SC, spinal cord and Sk, skin. (B) Quantification of the tissue distribution of the wild-type (open bars; n=8) and plc-1–/– (filled bars; n=12) ES cells. Relative levels of the GPI isozymes were quantified by densitometry software. Each column shows the percentage contribution of wild-type or plc-1–/– ES cells to the representative tissues isolated from the control and plc-1–/– chimeric mice and is expressed as the mean±s.e.m.
