Fig. 2. Paraffin and plastic sections of X-gal-stained mouse lungs 30 days after cell injection. (A) Low magnification (40x) and (B) high magnification (1000x) views of a representative recipient lung showing engrafted donor-derived cells as thin, flat blue cells consistent with a type I pneumocyte morphology. (C) For comparison, an X-gal-stained negative control lung (lacZ-negative marrow injected into a lacZ-negative recipient) shows no blue cells. (D) A thin plastic section (2 µm) of recipient mouse lung confirms the type I pneumocyte morphology of engrafted donor-derived cells. Sections are counter-stained with Nuclear Fast Red. Bars, 200 µm (A); 30 µm (B-D).
