Fig. 1. Perinatal expression of Gli and Shh in the mouse dorsal brain. (A,D,G) Expression of Gli1 (A), Gli2 (D) and Gli3 (G) in the vz/svz of the cerebral neocortex (Ctx) and striatum (Str) at embryonic day (E) 15.5. The three Gli genes are also co-expressed in the vz of the striatum and olfactory bulb (D and not shown). (B,E,H) Expression of Gli1 (B), Gli2 (E) and Gli3 (H) in the cerebral cortex (Ctx) at E17.5. Gli gene expression is detected near the ventricle (v) with Gli1 also expressed in scattered cells within the cortical plate. (C,F,I) Expression of Gli1 (C), Gli2 (F) and Gli3 (I) in the midbrain at E17.5. Expression of the Gli genes is regionalized in the vz of the tectum (Tct) and tegmentum (Tgt). Expression is also detected in the EGL and Purkinje layer of the cerebellum (Cb). Shh is also detected in the tegmentum as well as in the amygdala (not shown). (J) RT-PCR analyses of Shh expression in the parietal neocortex of E14.5-postnatal day (P)3 mice (right) and of its expression at E17.5 in the neocortex (Nctx), striatum (Str), superior colliculus (SC), inferior colliculus (IC) and cerebellum (Cb). A no RT control is also added (Nctx-RT). Hprt is used to control for RNA levels. (K-M) Shh is expressed in a layer-specific manner in the P2 neocortex (Ctx; K,L) and cingulate cortex (Cc; K), as well as in the tectum (Tct) and cerebellum (Cb), where it is found in the Purkinje layer. Shh is also expressed in the hippocampal dentate gyrus (Dg, also detected by RT-PCR, not shown). Similar expression was detected at P1 and P5. (N,O) Gli1 expression is found in the vz/svz of the cortex and in scattering cells (O) in a P5 mouse brain. Gli1 expression is also prominent in the dentate gyrus (Dg) of the hippocampus (Hip). Similar expression was detected at P1 and P5. (P) Expression of the oligodendrocyte precursor marker Pdgfr
in scattered cells and in a subset of cells near the ventricle. (Q-S) High magnification of the similar expression patterns of Gli1 (Q), Ptch1 (R) and Pdgfr (S) in scattering cells in the P5 cortex. Arrows point to sites of expression. v, ventricle. All panels show sagittal sections except N and the inset in K, which display coronal sections.
