Fig. 2. Abnormal patterns of gene expression in putative fusion-competent cells in minc mutant embryos. (A,B) Anti-Twist staining in wild-type (A) and minc[A388] (B) embryos at stage 14. Note maintained expression of twist in fusion-competent population in B. (C,D) Anti-Hairy in wild-type (C) and minc[A388] (D) embryos. Hairy is present in fusion-competent cells in C but absent from these cells in the mutant (D). Note hairy expression is unaffected in other tissues in these embryos. (E-H) RNA in situ hybridisations for sns and anti-ß-galactosidase staining in rP298;minc[A388]/TM3ftzlac (E,G) or rP298;minc[A388]/minc[A388] (F,H) embryos. Note loss of sns expression from somatic mesoderm in mutant embryos (F) but maintained presence in visceral mesoderm in the mutants (H). Arrows in G and H indicate midgut visceral mesoderm.
