Fig. 4. Embryonic pattern of minc expression. (A,B,E,F) RNA in situ hybridisations with the LD47926 probe. (C,D) as other panels but with anti-ß-galactosidase (brown) staining to reveal rP298 expression. (A) Embryo at late stage 10 showing minc expression in repeated clusters of cells corresponding to primordia of trunk visceral mesoderm. There is faint expression in mesodermal cross bridges below the clusters (arrowheads). (B) Stage 11 embryo showing minc expression in both founders (arrow) and fusion-competent cells (arrowhead) of visceral mesoderm. (C) Superficial (left) and deeper (right) planes of focus in stage 11 embryo to show transition from expression in visceral to expression in somatic fusion-competent cells. Expression in visceral mesoderm (arrows in right panel) is reduced as strong expression begins in somatic mesoderm (arrows in left panel). The location of the visceral mesoderm is indicated by the ribbon of rP298-expressing visceral founder cells. (D) Late stage 12 embryo: founder myoblasts express rP298 (arrows) but little if any minc; non rP298-expressing fusion-competent cells express minc at high levels (arrowheads). (E) Stage 13 embryo showing continued expression of minc in the somatic mesoderm. (F) By stage 15, minc expression is lost from the somatic mesoderm but now appears in the mesodermal cells of the gonad (arrow).
