Fig. 4. Expression of Eyc in pupae produces an eyc-like eye phenotype. (A) Electron micrograph of an adult eye of a Hs-GAL4/UAS-eyc fly exposed to six pulses of 45 minutes heat shock starting at 30% p.d. Approximately 23% of ommatidia in which Eyc was transgenically misexpressed show abnormal contact between rhabdomeres. (B) Heat shocks from 30% to 100% p.d. resulted in reduced rhabdomeres with abnormal contacts. Unshocked animals or parallel heat shocks to control flies did not produce rhabdomere defects. (C) Confocal micrographs of phalloidin-stained GMR-GAL4/UAS-eyc eyes show abnormal contacts of rhabdomeres (arrows). (D) Confocal micrograph of an Hs-GAL4/UAS-eyc eye whole-mount double-labeled with rhodamine-phalloidin (red) and 4C5 anti-Rh1 antibody (green). This animal received the same heat shock regimen as that in B. It shows abnormal rhabdomere adhesion in some photoreceptors (arrows). Deficient delivery of rhodopsin to the rhabdomere is also evident. Arrowheads indicate normal rhodopsin localization in some photoreceptors. Hs-GAL4 flies that received the same heat shock treatment do not show an eye phenotype. Scale bars: in A, 1 µm in A,B; in C, 5 µm in C,D.
