Fig. 7. Conserved sequences within the 1.3 kb MSE contain multiple candidate Oct6 binding sites. Two regions of highest sequence conservation between human and mouse fragment #5 (Fig. 6B) were analysed for candidate Oct6 binding sites. These consisted of 478 nt (domain A) and 436 nt (domain B) showing 84% and 90% sequence identity, respectively. Nucleotide numbering corresponds to fragment #5. Conserved residues are indicated with a dash in the human sequence. Nucleotide sequences corresponding to candidate Oct6 binding sites were identified by searching for sequences satisfying the Brn2 binding site consensus, C/AATnT/AAAT, where n=0,2,3 nt (Li et al., 1993). Brn2 and Oct6 are closely related class III POU proteins that are thought to exhibit similar binding characteristics (Schreiber et al., 1997). Conserved and non-conserved sites are overlined with a solid and hatched bar, respectively.
