Fig. 2. yps expression in ovaries. (A) A northern blot of ovary poly(A⁺)RNA probed with a yps cDNA. Two forms of the yps transcript are detected in wild-type ovaries (lane WT): a minor band of approximately 2.3 kb and a major broad band of approximately 1.7 kb, which may actually represent several transcripts of similar size. The positions of RNA molecular mass markers (M) are indicated on the right. In ovary poly(A⁺) mRNA from yps^JM2/Df(3L)BK9 females, the yps mRNAs are truncated and present at greatly reduced levels (lane yps^JM2). rp49 serves as a loading control for both samples. (B) Whole-mount in situ hybridization of a yps cDNA to wild-type ovaries. yps is expressed in the germ cells from early oogenesis on, and its transcripts are distributed evenly throughout the nurse cells and can be detected at a low level in the oocyte. yps is also expressed at a low level in the follicle cells. (C) A western blot of ovary protein, probed with an antibody to the N terminus of Yps (a region that includes the CSD) reveals a single protein isoform migrating at approximately 50 kDa (lane WT). In protein extracted from yps^JM2/Df(3L)BK9 ovaries (lane yps^JM2), Yps is not detected.

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