Fig. 7. Orb coimmunoprecipitates with Exu and Yps in an RNAse-sensitive manner. (A) Immunoblot for the presence of Orb in Exu and Yps immunoprecipitates from GFP-Exu extracts using anti-GFP (GFP), anti-Yps (YPS) or rabbit IgG antibodies. Orb protein specifically coimmunoprecipitates with both Exu and Yps. (B) Immunoblot for the presence of Orb in Exu and Yps immunoprecipitates from RNAse-treated GFP-Exu extracts using anti-GFP (GFP), anti-Yps (YPS) or rabbit IgG antibodies. Orb protein does not coimmunoprecipitate with either Exu or Yps when RNAse-treated extracts are used, indicating that RNA is required for Orb to associate with Exu and Yps. In both panels, the right lane shows ovary extract prior to immunoprecipitation.
