Fig. 5. (A) Schematic representation of the experimental procedure. Regions 1, 2, 3, 4 and 5 represent neck level- (yellow), wing level- (turquoise), flank level- (blue) and leg level- (pink) median tissues of stage 12 and the presumptive leg region of stage 10 (pink), respectively. (B) Tbx5/Tbx4 expression in cultured explants. Lanes 1, 2, 3 and 4 represent Tbx5/Tbx4 expression of each level-median tissue when regions 1, 2, 3 and 4 were cultured separately. Lanes 1+5, 2+5, 3+5 and 4+5 represent Tbx5/Tbx4 expression of each level-median tissues and the presumptive leg region when these two regions were co-cultured. Forty-eight hours after incubation, RT-PCR and Southern blot analyses were performed. The relative amount of expression of each gene was plotted (C,D) after being standardized with ß-actin. The amount of each Tbx gene expression in 2+5 explants was taken as 1.0. Each experiment was repeated 5 times. Error bars indicate s.d..
