Fig. 1. daf-9 mutant phenotype. (A) C. elegans hermaphrodite reproductive system. The gonad consists of anterior and posterior U-shaped tubular arms, with ovaries connecting to ventral spermathecae, which then join to the uterus, terminating at the vulva. (B) Normal morphology in a daf-9(e1406) daf-12(m20) double mutant. (C) Abnormal reproductive system of a daf-9(e1406) adult. Vesicles are present in much of the space normally occupied by the uterus. A large pseudovulval protrusion is shown. (D) Abnormal distal tip cell migration in daf-9(e1406). The anterior gonad arm did not reflex dorsally, but extended anteriorly to the pharynx. (E) Molting defect of daf-9(e1406). A shed anterior cuticle remains attached to the body, resulting in a constriction. rg, dorsal ovary of the reflexed gonad; vul, vulva; pv, pseudovulval protrusion; dtc, distal tip cell; v, vesicle; ac, shed anterior cuticle; *, constriction. Scale bars: 10 µm.
