Fig. 3. Neural stem cell colonies from E14.5 medial ganglionic eminence germinal zone show a unique migratory potential. Isolated neural stem cell colonies derived from cortical (CTX) or medial ganglionic eminence (MGE) germinal zone of GFP transgenic mice were deposited on CD1 coronal slices and observed under phase contrast (A) and fluorescent (B) microscopy. After 48 hours co-culturing, cells migrating out of sphere colonies derived from CTX (C) or MGE (D) were observed using fluorescence microscopy. (E) Higher magnification image of the boxed area in D is shown and the numbers of cells that had migrated into the three 0.25 mm x 0.25 mm areas indicated (I-III) were counted. Distance migrated: I, 0.25-0.5 mm; II, 0.5-0.75 mm; III, 0.75-1.0 mm. (F) The number of cells in zones I, II or III (derived from CTX (n=9) or MGE (n=12) sphere colonies after 48 hours co-culturing with CD1 coronal slice) were quantified. Scale bar, 0.5 mm (A-D).
