DEV2792 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 β-cat -

  β-catenin staining during stages 8-31 of Xenopus development.
  Most images present both original and nuclear images (masks), as described in the manuscript. For β-catenin staining of the latest stages, we show only merged β-catenin/DAPI images. Nuclear images could not be obtained due to poor separation of the nuclear and membrane signals in these small cells.
  Abbreviations: ac, archenteron; ba, branchial arches; ax, axial mesoderm; bl, blastocoel, bp, dorsal blastopore; br, brain; bv, brain vesicle; cc, circumblastoporal collar; cg, cement gland; dm, dermatome; dl, dorsal lip; en, endoderm; ev, ear vesicle; ey, eye/ eye anlage; fb, forebrain; fg, foregut; fi, dorsal fin; ha, heart anlage; hb, hindbrain; mb, midbrain; me, mesenchyme; mg, midgut; ne, neurectoderm; nc, neural crest cells; no, notochord; nt, neural tube; pn, pronephros anlage; pr, proctodeum; sm, presomitic/ somitogenic mesoderm; so, somites; st, stage; tb, tailblastema/tailbud; vl, ventral lip; vm, ventral mesoderm.

• Supplemental Figure 1 MAPK -

  MAPK staining during stages 8.5-31 of Xenopus development.
  Most images present both original and nuclear images (masks), as described in the manuscript.
  Abbreviations: ac, archenteron; ant, anterior; ba, branchial arches; ax, axial mesoderm; bl, blastocoel, bp, dorsal blastopore; br, brain; bv, brain vesicle; cc, circumblastoporal collar; cg, cement gland; dm, dermatome; dl, dorsal lip; en, endoderm; ev, ear vesicle; ey, eye/ eye anlage; fb, forebrain; fg, foregut; fi, dorsal fin; ha, heart anlage; hb, hindbrain; mb, midbrain; me, mesenchyme; mg, midgut; ne, neurectoderm; nc, neural crest cells; no, notochord; np, neural plate; nt, neural tube; pn, pronephros anlage; pr, proctodeum; sm, presomitic/ somitogenic mesoderm; so, somites; st, stage; tb, tailblastema/tailbud; vl, ventral lip; vm, ventral mesoderm.

• Supplemental Figure 1 Smad1 -

  Smad1 staining during stages 8.5-31 of Xenopus development.
  Most images present both original and nuclear images (masks), as described in the manuscript. Note also that the epidermis of late stage embryos shows strong cross-reactivity with Smad1 and Smad2 antibodies.
  Abbreviations: ac, archenteron; ant, anterior; ba, branchial arches; ax, axial mesoderm; bl, blastocoel, bp, dorsal blastopore; br, brain; bv, brain vesicle; cc, circumblastoporal collar; cg, cement gland; dm, dermatome; dl, dorsal lip; en, endoderm; ev, ear vesicle; ey, eye/ eye anlage; fb, forebrain; fg, foregut; fi, dorsal fin; ha, heart anlage; hb, hindbrain; mb, midbrain; me, mesenchyme; mg, midgut; ne, neurectoderm; nc, neural crest cells; no, notochord; np, neural plate; nt, neural tube; pn, pronephros anlage; pr, proctodeum; sm, presomitic/ somitogenic mesoderm; so, somites; st, stage; tb, tailblastema/tailbud; vl, ventral lip; vm, ventral mesoderm.

• Supplemental Figure 1 Smad2 -

  Smad2 staining during stages 8-31 of Xenopus development.
  Most images present both original and nuclear images (masks), as described in the manuscript. Note also that the epidermis of late stage embryos shows strong cross-reactivity with Smad1 and Smad2 antibodies.
  Abbreviations: ac, archenteron; ant, anterior; ba, branchial arches; ax, axial mesoderm; bl, blastocoel, bp, dorsal blastopore; br, brain; bv, brain vesicle; cc, circumblastoporal collar; cg, cement gland; dm, dermatome; dl, dorsal lip; en, endoderm; ev, ear vesicle; ey, eye/ eye anlage; fb, forebrain; fg, foregut; fi, dorsal fin; ha, heart anlage; hb, hindbrain; mb, midbrain; me, mesenchyme; mg, midgut; ne, neurectoderm; nc, neural crest cells; no, notochord; np, neural plate; nt, neural tube; pn, pronephros anlage; pr, proctodeum; sm, presomitic/ somitogenic mesoderm; so, somites; st, stage; tb, tailblastema/tailbud; vl, ventral lip; vm, ventral mesoderm.

• Supplemental Figure 2A -

  β-catenin (Powerpoint Animation)

• Supplemental Figure 2B -

  MAPK (Powerpoint Animation)

• Supplemental Figure 2C -

  Smad1 (Powerpoint Animation)

• Supplemental Figure 2D -

  Smad2 (Powerpoint Animation)