Fig. 2. Immunolocalization of TIF1ß in post-pubertal wild-type testis. Histological sections from paraffin wax-embedded testes were incubated with the anti-TIF1ß antibody, whose binding to cell structures was then revealed with a Cy3-conjugated secondary antibody (red signal) and nuclei were counterstained with DAPI (blue signal). BrdU incorporation in the tubular cross section shown in C was detected using a green fluorochrome. Roman numerals refer to stages of the seminiferous epithelium cycle. ES-10 and ES-16 correspond to steps in spermatid maturation. L, leptotene spermatocytes; LU, lumen of the seminiferous tubules; P, pachytene spermatocytes; PR, preleptotene spermatocytes; RS, round spermatids; S, Sertoli cells; SG, spermatogonia. Scale bar: 25 µm in A,B; 55 µm in C.
