Fig. 5. Histological aspect of TIF1ß(^L2/L2):PrP-Cre-ER^T(0/0) control testis (A,C) and TIF1ß^L2/L2:PrP-Cre-ER^T(tg/0) experimental testis (B,D-H), 8 weeks after tamoxifen treatment. The seminiferous tubule in C is at stage VII. The abnormal seminiferous tubules in D,G are difficult to stage because of the complete absence of spermatocyte populations; however, they have features in common with a stage VII tubule (‘VII’), namely the aspect of acrosomes in round spermatids (not visible at this magnification) and/or alignment of elongated spermatids. Groat Hematoxylin and Malloy’s trichrome (A-F) or immunolabeling for TIF1ß and DAPI counterstaining (G,H). L, Leydig cells; NT, tubules showing normal germ cell associations; P, pachytene spermatocytes; PR, preleptotene spermatocytes; RS, round spermatids; S, Sertoli cells; ST, Sertoli cell-only seminiferous tubules. The arrowhead in E indicates exfoliated round spermatids. Scale bar: 100 µm (A,B), 15 µm (C,D,F) and 20 µm (E,G,H).

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