Fig. 3. Loss of AmpA function delays differentiation of prestalk cells and migration of stalk A cells from the mound periphery to the mound tip. EcmA reporter expression patterns are shown for wild-type structures (left) and ampA– structures (right) for the following developmental stages: (A,B), tight mounds, 10 hours of development; 2 hours staining. (C,D), late tight mounds, 12 hours of development; 1 hour staining. (E,F), tips, 13.5 hours of development; 1 hour staining. (G,H) Culminants, 22 hours of development, 1 hour staining. Arrows indicate the mound periphery where stalk A cells initially differentiate.
