Fig. 5. Inactivation of ampA gene function results in a premature increase in the percentage of cells expressing the ampA reporter at the earliest developmental stages. Axenically growing wild-type (A) and ampA– (B) cells carrying the ampA reporter gene were washed free of growth medium, plated for development on nitrocellulose filters and stained 19 hours for expression of ß-galactosidase after 4 hours of development as described in the Materials and Methods. (C) Quantification of the percentage of cells expressing the ampA reporter in developing wild-type and ampA– structures during development on nitrocellulose filters. Structures harvested at 12 hours of development were dispersed to single cells, and fixed, stained and counted as described in the Materials and Methods.
